Fig 5B: Output from the AACompIdent tool where a protein from an S.
cerevisiae 2-D gel was correctly identified by matching its amino acid
composition, estimated pI and MW and an N-terminal sequence tag of XYTT
against database entries for S. cerevisiae. Protein sequence tag and amino
acid composition were determined according to Wilkins et al. (11). The
correct protein identity, inorganic pyrophosphatase, was the top-ranked
protein in all three lists and was the only protein to show the sequence tag
at its predicted N-terminus. Note that when the sequence tag is found
anywhere in the protein it is indicated by an asterisk to the left of the
protein rank, and the tag is shown in lower case if found in the displayed
sequence. The AACompIdent output was shortened for this figure.
------------------------------------------
SEARCH VALUES:
Constellation 2
Calibration protein: ALBU_BOVIN ( P02769 )
Species searched: YEAST
Name given to unknown protein: YEASTJI7
Tagging: YTT
The N-terminal sequence of the protein will be printed.
------------------------------------------
SpotNb YEASTJI7
===============
pI: 5.25 Range: ( 4.75, 5.75)
Mw: 32018 Range: ( 25614, 38422)
The closest Swiss-Prot entries for the species YEAST:
Rank Score Protein (pI Mw) N-terminal Sequence
=========================================================
* 1 19 IPYR_YEAST 5.36 32184 TyttRQIGAKNTLEYKVYIEKDGKPVSAFHDIPLYADKEN
2 23 YCY4_YEAST 7.59 44982 MVSLFKRGKAPPLTKEGPTSKKPPNTAFRQQRLKAWQPIL
3 30 YJF4_YEAST 5.61 54182 MSSESGKPIAKPIRKPGYTNPALKALGIPALRLPSRNWMI
* 4 30 SYV_YEAST 5.95 119994 SDLDNLPPVDPKTGEVIINPLKEDGSPKTPKEIEKEKKKA
5 30 YJG0_YEAST 6.17 50082 MKQRFIRQFTNLMSTSRPKVVANKYFTSNTAKDVWSLTNE
The closest Swiss-Prot entries:
Rank Score Protein (pI Mw) N-terminal Sequence
=========================================================
* 1 19 IPYR_YEAST 5.36 32184 TyttRQIGAKNTLEYKVYIEKDGKPVSAFHDIPLYADKEN
2 23 YCY4_YEAST 7.59 44982 MVSLFKRGKAPPLTKEGPTSKKPPNTAFRQQRLKAWQPIL
3 24 MTMU_MYCSP 9.04 26933 MENKVTAYSIYNKKAKKNTKVNPLDEVFPQLPRKKYQVIY
4 26 PUR7_CANMA 5.09 32941 MTSTNLEGTFPLIAKGKVRDIYQVDDNTLLFVATDRISAY
* 5 26 RAC1_DICDI 6.82 21573 MQAIKCVVVGDGAVGKTCLLISyttNAFPGEYIPTVFDNY
The Swiss-Prot entries having pI and Mw values in the specified range:
Rank Score Protein (pI Mw) N-terminal Sequence
=========================================================
* 1 19 IPYR_YEAST 5.36 32184 TyttRQIGAKNTLEYKVYIEKDGKPVSAFHDIPLYADKEN
2 36 MT16_YEAST 5.62 29882 MKTYHLNNDIIVTQEQLDHWNEQLIKLETPQEIIAWSIVT
3 38 YNK7_YEAST 5.26 25981 MAPTISKRIKTLSVSRPIIYGNTAKKMGSVKPPNAPAEHT
* 4 44 MM21_YEAST 5.02 30340 MALNDNPIPKSVPLHPKSGKYFHNLHARDLSNIYQQCYKQ
5 45 YGF3_YEAST 5.01 27250 MQTPSENTNAKSDSLDEPGAYLIEENVALPKDIFHSYLSY
------------------------------------------
Last modified 22/Jan/2003 by ELG
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