HhaI Methylase "F13" Structure

Color the protein segments: Large domain (cyan) with "active site loop" (green); Small domain (yellow); and hinge (orange).

DNA site: Nucleotides in the GCGC recognition sequence are "Sticks" with CPK colors; labeled at the phosphates. The protein is "Ribbons" with "Structure" colors.

Amino acids that interact with the DNA bases are "Ball&Stick" colored CPK; and labeled at their C-a's.

Amino acids that interact with the DNA backbone are "Ball&Stick" colored CPK; and labeled at their C-a's.

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Viewing Tips:
1. Loading image (1mht.pdb) is similar to Fig. 1 of Roberts & Cheng (1998) "Base flipping". Annu. Rev. Biochem. 67 181-198.
2. The highlight buttons are based on the listing in Table 1 of Klimasauskas et al. (1994).
3. Use the Rotate buttons to generate additional views of the complex, e.g. Figs. 2-4 of Klimasauskas et al.. Zoom (Shift-left and drag) for close-ups of the DNA-protein interactions, e.g. Figs. 5-7 & 9.

Coordinate file: 1mht.pdb Reference: Klimasauskas et al. (1994) "HhaI methyltransferase flips its target base out of the DNA helix." Cell 76 357-369.

Ten additional structures can be viewed on a Chime page, Structures of DNA-Base Flipping Proteins, in a side-by-side format.

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