FEBS Lett 1998 Jul 3;430(3):251-6

Functional analyses of the Dof domain, a zinc finger DNA-binding domain, in a pumpkin DNA-binding protein AOBP.

Shimofurutani N, Kisu Y, Suzuki M, Esaka M

Faculty of Applied Biological Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan.

AOBP, a DNA-binding protein in pumpkin, contains a Dof domain that is composed of 52 amino acid residues and is highly conserved in several DNA-binding proteins of higher plants. The Dof domain has a significant resemblance to Cys2/Cys2 zinc finger DNA-binding domains of steroid hormone receptors and GATA1, but has a longer putative loop where an extra Cys residue is conserved. We show that the Dof domain in AOBP functions as a zinc finger DNA-binding domain and suggest that the Cys residue uniquely conserved in the putative loop might negatively regulate the binding to DNA.

PMID: 9688549, UI: 98351835

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