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Protein families database of alignments and HMMs
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Pfam Protein families database of alignments and HMMs Home | Keyword search | Protein search | DNA search | Browse Pfam | Taxonomy search | Help |
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| Accession number: PF00036 |
| EF hand |
| The EF-hands can be divided into two classes: signaling proteins and buffering/transport proteins. The first group is the largest and includes the most well-known members of the family such as calmodulin, troponin C and S100B. These proteins typically undergo a calcium-dependent conformational change which opens a target binding site. The latter group is represented by calbindin D9k and do not undergo calcium dependent conformational changes. |
IPR002048)Many calcium-binding proteins belong to the same evolutionary family and share a type of calcium-binding domain known as the EF-hand. This type of domain consists of a twelve residue loop flanked on both side by a twelve residue alpha-helical domain. In an EF-hand loop the calcium ion is coordinated in a pentagonal bipyramidal configuration. The six residues involved in the binding are in positions 1, 3, 5, 7, 9 and 12; these residues are denoted by X, Y, Z, -Y, -X and -Z. The invariant Glu or Asp at position 12 provides two oxygens for liganding Ca (bidentate ligand). |
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[1] Evolution of EF-hand calcium-modulated proteins. II. Domains of several subfamilies have diverse evolutionary histories. Nakayama S, Moncrief ND, Kretsinger RH; J Mol Evol 1992;34:416-448. |
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[2] Comparison of terbium (III) luminescence enhancement in mutants of EF hand calcium binding proteins. Hogue CW, MacManus JP, Banville D, Szabo AG; J Biol Chem 1992;267:13340-13347. |
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[3] EF-hand motifs in inositol phospholipid-specific phospholipase C. Bairoch A, Cox JA; FEBS Lett 1990;269:454-456. |
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[4] The evolving model of calmodulin structure, function and activation. Finn BE, Forsen S; Structure 1995;3:7-11. |
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| PROSITE | PDOC00018 [Expasy|SRS-UK|SRS-USA] |
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| PROSITE | PDOC00275 [Expasy|SRS-UK|SRS-USA] |
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| INTERPRO | IPR002048 |
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| PFAMB The following Pfam-B families contain sequences that according to Prodom are members of this Pfam-A family. |
PB013105 PB014587 PB017810 PB017856 PB027852 PB030994 PB038856 PB038981 PB039287 PB039338 PB039476 PB039500 PB039817 PB039971 |
| Author of entry | Eddy SR |
| Alignment method of seed | Clustalw |
| Source of seed members | Prosite |
| Gathering cutoff | 16.8 1 |
| Trusted cutoff | 17.00 1.00 |
| Noise cutoff | 16.70 16.70 |
| Build method of HMM | hmmbuild HMM SEED hmmcalibrate --seed 0 HMM |
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